Figure 6.

PFF-induced α-synuclein phosphorylation and propagation of α-synuclein aggregation

(A) Wild-type mice (3 months old at time of injection) were injected with PFFs to the striatum (STR) and stained for α-synuclein phosphorylated at S129 (p-aSyn) in the STR and substantia nigra (SN) areas at 1, 3, and 6 months after PFF injection. Images at 2.5× magnification and close-ups at 40× magnification are shown. Black arrows at 2.5× indicate approximate areas of p-aSyn-positive staining (dark brown); 40× images show widespread p-aSyn-positive staining. (B) Double immunofluorescence staining of substantia nigra area for tyrosine hydroxylase (TH, red) and p-aSyn (green) of injected (left panels) and uninjected hemispheres (right panels). Scale bars represent 200 μm (top row) and 50 μm (bottom rows). Additional controls are included in Figure S9.