Figure 2.

AAV transduction in CNS areas as a function of injection route

(A) Unsupervised clustering analysis of the viral loads (expressed as the number of viral genome copies per cell) in all brain areas analyzed, irrespective of the specific capsid barcode. (B) Quantification of ddPCR analysis of the viral loads in brain areas after CSF (ICM, ICV, or IT) library administration. (C) Quantification of ddPCR analysis of the viral loads in brain areas after intra-putamen virus administration (the samples not shown: Occ, DCN, Cer, and PSC for one animal were below the threshold sensitivity and were not plotted). Of note, the front lobe (Fro) data were excluded from the clustering analysis because the respective sample was not collected for the IP injection route. In addition, ipsilateral putamen sample data from both IP-injected animals were excluded as well because their viral DNA content exceeded all other samples by two to four orders of magnitude (Figure 2C), making it an outlier for the purpose of the clustering analysis.