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Infectious attenuated viral strains rescued from RNA-transfected Vero cells
Rescued virus strains were passaged twice, and the level of attenuation relative to CHIKV-LR was measured by growth curves (MOI of 0.01) on Vero cells. (A and B) Supernate virus content was measured by qRT-PCR of viral genomes (A) or plaque assay (B). Data points represent mean values from biological triplicate samples ± SEM.
