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Zebrafish embryos exposed to kratom decoction, mitragynine, speciociliatine, and morphine. (A) hatching rate at 72 and 96 hpf and (B) heart rate per minute. Data are expressed as the mean ± SD *p < 0.05, **p < 0.01, ***p < 0.001 significantly different from the negative control group. # indicate not determined.
