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. 2021 Aug 20;12:632045. doi: 10.3389/fphar.2021.632045

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Copyright © 2021 Li, Zhao, Gu, Chen and Yu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The safety of long-term use of H₂-rich water and its effects on quality of life of rats. (A) Healthy individuals were given regular water (N) and H₂-rich water (H₂) for 9 months and sacrificed for the detection of pathologic morphology of heart, kidney, and liver. (B) The levels of AST and ALT in two groups. (C) The hair and fur were observed in these healthy individuals. Hematoxylin and eosin staining (HE) and immunohistochemistry (Ki67) showed more hair shaft (the fiber) and hair bulb (the root) in rats receiving H₂-rich water than those without drinking H₂-rich water. (D) The concentration and motility of sperm of these rats. *p < 0.05. HE, IHC × 200.