FIG. 5.

Aryl azide derivatization of 12-RSS substrates. Aryl azide moieties (arrows) were coupled to one of three pairs of phosphorothioates (S15-S16, S20-S21, and S26-S27) in the heptamer regions of WT or MT 12-RSS substrates, as defined in Materials and Methods (the wild-type substrate is depicted here). Specific RAG-DNA contacts, as defined by ethylation interference (circles) and methylation or KMnO₄ interference (triangles), are marked. Putative RAG-induced structural perturbations in substrate DNA, as defined by overrepresentation of KMnO₄ modification, are indicated by diamonds. Open symbols represent interactions detected in 12-RSS complexes containing RAG-1 alone; closed and open symbols, taken together, denote interactions detected in complexes containing RAG-1 and RAG-2.