TABLE 3.
Virulence gene and regulator genes of ctx in the strains analyzed in this study.
| Identity (%) | |||
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| Strain No. 1 | Strain No. 2 | Strain No. 3 | |
| Gene (accession No. and locus of reference gene) | |||
| ctxAB (NC_002505, VC1456, 1457) | 99.8% (1146/1148) | 99.7% (1144/1148) | 99.7% (1144/1148) |
| tcpA (NC_002505, VC0828) | 75.3% (508/675) | 74.2% (501/675) | 76.7% (518/675) |
| zot (NC_002505, VC1458) | 99.0% (1188/1200) | 98.9% (1187/1200) | 99.1% (1189/1200) |
| ace (NC_002505, VC1459) | 98.0% (288/294) | 99.3% (292/294) | 99.3% (292/294) |
| hapA (NC_002506, VCA0865) | 98.3% (1799/1830) | 99.1% (1814/1830) | 100.0% (1830/1830) |
| hlyA (NC_002506, VCA0219) | 97.3% (2167/2226) | 98.2% (2185/2226) | 99.96% (2225/2226) |
| rtxA (NC_002505, VC1451) | Deletion * | 98.4% (13460/13677) | Deletion ** |
| chxA (NZ_KQ4110624, A55_RS07670) | N.D. | N.D. | N.D. |
| stn (M85198 M36061) | N.D. | N.D. | N.D. |
| TTSS (accession No. of reference gene: DQ124262) | |||
| Gene of component of TTSS (locus tag) | |||
| VcsN2 (NT01VC2327) | 99.2% (1253/1263) | 99.1% (1252/1263) | N.D. |
| VcsC2 (NT01VC2328) | 98.8% (1440/1458) | 99.5% (1450/1458) | N.D. |
| VcsT2 (NT01VC2330) | 98.7% (675/684) | 98.8% (676/684) | N.D. |
| VcsR2 (NT01VC2331) | 96.9% (654/675) | 98.7% (666/675) | N.D. |
| VcsQ2 (NT01VC2334) | 98.1% (212/216) | 98.1% (212/216) | N.D. |
| VcsU2 (NT01VC2339) | 99.3% (1046/1053) | 99.3% (1046/1053) | N.D. |
| VcsV2 (NT01VC2340) | 99.2% (1869/1884) | 99.5% (1874/1884) | N.D. |
| VspD (NT01VC2345) | 99.1% (1041/1050) | 99.3% (1043/1050) | N.D. |
| VcsJ2 (NT01VC2348) | 97.9% (523/534) | 97.9% (523/534) | N.D. |
| Regulatory gene of ctx (accession No. and locus of reference gene) | |||
| toxT (NC_002505, VC0838) | 100% (831/831) | 98.8% (821/831) | 98.8% (821/831) |
| toxRS (NC_002505, VC0826, VC0827) | 99.5% (1409/1416) | 98.6% (1396/1416) | 100% (1416/1416) |
| tcpPH (NC_002505, VC0983, VC0984 | 99.2% (1053/1061) | 98.0% (1040/1061) | 97.7% (1037/1061) |
| hapR (MF099991) | 99.4% (647/651) | 99.5% (648/651) | 99.2% (646/651) |
N.D., Not detected DNA fragment whose sequence was homologous to that of the reference gene. *, The region covering from nucleotide 2,603 to 11,789 of the reference sequence was lost in the gene of the corresponding strain. **, The regions covering from nucleotides 3,028 to 3,198 and 11,332 to 13677 of the reference sequence was lost in the gene of the corresponding strain.