Figure 6.

CDR3 sequences found in tumor-infiltrating lymphocytes correlates with expansion of T cells in a patient after doxorubicin and cyclophosphamide (NAC-AC) plus dendritic cell (DC) treatment. (A) Similarity analysis of the CDR3 sequence by Morisita index 0–1 (no similarity to complete similarity, respectively) represented in a heat map. (B) Venn diagram showing the number of overlapping rearrangements present in the four sequenced samples [tumor, lymph node, and peripheral blood mononuclear cells (PBMCs) before and after NAC+DC vaccination]. (C) Scatter dot plot comparing the frequency of unique sequences with significant differences (increased or decreased) between samples (orange and blue dots), excluded sequences [white dots, below threshold (orange dashed line)] and nonsignificant differences [gray dots, near frequency equality (black dashed line)]. (D) Paired comparison of the frequency of shared rearrangements among the three samples (n = 182), PRE (black dots), VAC (blue dots), and tumor (red dots). Top CDR3 sequences were denoted alongside the tumor CDR3 rearrangement. Parametric one-way ANOVA test with Turkey’s multiple comparison test among the three samples, ****p < 0.0001.