FIGURE 3.

Angiotensin II-induced hypertension promotes neuronal apoptosis in middle-aged mice. (A) Representative images of TUNEL-positive, fluorescein-labeled (green) neurons co-stained with the anti-NeuN antibody (red) in the cortex and hippocampus (CA1, DG, and CA3) of mice in the control and hypertension groups (immunofluorescence, ×20, scale bar = 50 μm). (B) Histograms comparing the level of positive TUNEL staining in neurons in the cortex and hippocampus (CA1, DG, and CA3) of mice in the control and hypertension groups. Data are expressed as the means ± SD. * p < 0.05. (Negative control image of TUNEL staining assay provided in Supplementary Figure 3).