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. 2021 Aug 20;13:716917. doi: 10.3389/fnagi.2021.716917

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Copyright © 2021 Xu, Du, Jiang, Yang, Wang, Wang, Tang, Fu and Hao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Concentrations of IL-1α, TNF-α, and C1q were tested in MCM, Triggering receptor expressed on myeloid cells 2 (TREM2) (vector or overexpression) transfection-conditioned mediums, and TREM2 (vector or overexpression) + MCM. The concentration levels were measured using ELISA kits. (A) Concentration of TNF-α. (B) Concentration of IL-1α. (C) Concentration of C1q. Differences between the means were determined by t test. *p < 0.05 versus control at the match time point, ** p < 0.05 versus LPS (0.1 ug/m) at the match time point. The data are expressed as means ± SEM from three independent experiments. C1q: complement component 1q; IL-1α: interleukin 1α; TNF-α: tumor necrosis factor-α.