Fig. 3.
ATG5 knockdown suppresses the inhibitory effects of PS-T. a MDA-MB-231-siNC cells and MDA-MB-231-siATG5 cells are treated with or without PS-T (5 μg/mL) for 24 h. The levels of each EMT marker in MDA-MB-231cells are quantified using NIH ImageJ software. (mean ± SD, *P < 0.05 and **P < 0.01). b The invasiveness of MDA-MB-231-siNC cells and MDA-MB-231-siATG5 cells treated with PS-T at 5 μg/mL is evaluated using Transwell invasion assays at 24 h (mean ± SD, **P < 0.01). c, d Migratory ability of MDA-MB-231-siNC and MDA-MB-231-siATG5 cells treated with PS-T at 5 μg/mL is evaluated using Transwell migration assays (c) and scratch assay (d) at 24 h (mean ± SD, *P < 0.05, **P < 0.01, and ***P < 0.001)