FIGURE 2.

VEGF-A protein levels in Ins2 ^Akita mice at different ages. (A): Representative western blottings of VEGF-A₁₆₄ and the respective α-tubulin, measured in the same samples, of total homogenates of retinae from Ins2 ^Akita (AK) mice at different ages (w = weeks). (B): Densitometric analysis of VEGF-A₁₆₄ immunoreactivities in the total homogenates of retinae from wild-type and AK mice at different ages. Alpha-tubulin was used as a loading control. Results are expressed as % (±S.E.M.) vs control wild-type (100%, dashed line). *p < 0.05, **p < 0.01 vs wild-type; Tukey’s Multiple Comparison post-hoc test, n = 8–10.