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. 2021 Aug 13;12:693118. doi: 10.3389/fimmu.2021.693118

Figure 2.

Figure 2

In vivo syngeneic mouse model of EM. (A) Treatment regimen of C3 WT and gene-deficient mice for generating EM in vivo model. Five C3-/- and WT mice each were injected (i.p.) with minced uterus of a donor mouse C3-/- and WT respectively; after 3 weeks, the peritoneal cyst formation was evaluated. (B) Number of cysts counted in wild-type (WT) mice injected with WT endometrium (n = 5) or C3-/- injected with C3-/- endometrium (n = 5). Mann-Whitney test p = 0.03. (C, D) Representative images of cytocentrifuged peritoneal washing of untreated WT, EM-induced WT and C3-/- mice (respectively), stained with Giemsa and counted with ImageJ software (Particle Analysis Tool) to obtain relative percentage between total leukocytes and mast cell (MC)/basophil number. MCs/basophils are identified as blue big dots indicated by red arrows. Original magnification 100×. (E) Representative images of cytocentrifuged peritoneal lavage of EM-induced WT mice stained with FITC-conjugated anti-mouse CD117. Original magnification 100×. (F) Biochemical characterization of tryptase enzyme present in peritoneal lavage of WT vs C3-/- mice by ELISA. *p < 0.05.