Figure 3.

HRW administration alleviates oxalate-induced renal fibrosis in mice. (A) The degree of renal collagen was assessed with Masson trichrome staining (Scale bar = 50 μm); The protein expressions of α-SMA, FN, and COL IV in kidneys of mice were detected by immunohistochemistry (Scale bar = 100 μm). (B–E) Corresponding semiquantitative analysis of Masson trichrome staining and α-SMA, FN and COL IV immunohistochemistry staining. (F) The protein expressions of α-SMA, FN and COL IV in kidneys of mice were detected by western blotting. The results were expressed as mean ± SEM. Statistical comparisons were performed using a NewmanKeuls test (^*p < 0.05 vs. ND group, ^#p < 0.05 vs. OD group).