Figure 5.

HRW administration suppresses oxalate-induced PI3K/AKT signaling pathway activation in mice. (A) Heatmap and hierarchical clustering showing DEGs enriched in the PI3K/AKT signaling pathway between the OD and ND groups. (B) PPI network constructed by DEGs enriched in the PI3K/AKT signaling pathway. (C) The protein expressions of PI3K, AKT, and p-AKT in kidneys of mice were detected by western blotting. (D) The protein expressions of PI3K and p-AKT in kidneys of mice were detected by immunohistochemistry (Scale bar = 100 μm). (E,F) Corresponding semiquantitative analysis of PI3K and p-AKT immunohistochemistry staining. The results were expressed as mean ± SEM. Statistical comparisons were performed using a NewmanKeuls test (*p < 0.05 vs. ND group, ^#p < 0.05 vs. OD group).