Figure 6.

HRW administration suppresses oxalate-induced NF-κB signaling pathway activation in mice. (A) Heatmap and hierarchical clustering showing DEGs enriched in the NF-κB signaling pathway between the OD and ND groups. (B) PPI network constructed by DEGs enriched in the NF-κB signaling pathway. (C) The protein expressions of NF-κB p65, p-NF-κB p65, NLRP3 and IL-1β in kidneys of mice were detected by western blotting. (D) The protein expressions of NF-κB p65, p-NF-κB p65, NLRP3 and IL-1β in kidneys of mice were detected by immunohistochemistry (Scale bar = 100 μm). (E–H) Corresponding semiquantitative analysis of NF-κB p65, p-NF-κB p65, NLRP3, and IL-1β immunohistochemistry staining. The results were expressed as mean ± SEM. Statistical comparisons were performed using a NewmanKeuls test (^*p < 0.05 vs. ND group, ^#p < 0.05 vs. OD group).