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. 2021 Aug 19;11:657984. doi: 10.3389/fonc.2021.657984

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Copyright © 2021 Hu, Chen, Guo, Li, Fu, Chen, Zhao, Wei, Li and Wu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of miR-425 and miR-576 on tumor metastasis by inhibiting PTEN-P53/TGF-β axis in CRC Cells. (A, B) The levels of PTEN mRNA after miR-425/miR-576 overexpression (A) or knockdown (B) in HCT116 and SW480 cells detected by qPCR assay. (C, D) The changes in PTEN protein and P53 and TGF-β upon interfering miR-425 and/or miR-576 expression in HCT116 and SW480 cells detected by Western blot assay. (E) Dual-Luciferase reporter assay identifying the specific binding activities of PTEN 3′UTR with miR-425/miR-576 in HCT116 and HEK293T cells. (F–I) The cell migration abilities in CRC cells treated with mimics and inhibitors of miR-425 and/or miR-576 detected by cell migration assay. All tests were performed at least three times. Data were expressed as mean ± SD (ns, p > 0.05; ***p < 0.001; ****p < 0.0001).