Figure 9.

Multifunctional aptamer-linked DNA-Au nanomachine (Apt-DNA-Au) (A) TEM and (C) CLSM images of the states of Au NPs in MCF-7 cells, HeLa cells, and L02 cells, respectively, after incubation with Cy5‐labeled Apt‐DNA‐Au nanomachine. Scale bar: 100 μm. (B) The results of different expression levels of TK1 mRNA in MCF-7 cells by flow cytometry. Scale bar: 100μm. (D) In vivo fluorescence imaging of Apt-DNA-Au nanomachine at different times. (E) In vivo fluorescence imaging of organs and tumors treated with saline (top) and Apt-DNA-Au nanomachines (bottom). (F) The signal intensity of different organs and tumors. Reproduced from Yu S, Zhou Y, Sun Y, et al. Endogenous mRNA Triggered DNA–Au Nanomachine for in Situ Imaging and Targeted Multimodal Synergistic Cancer Therapy. Angew Chemie Int Ed. 2021;60(11): 5948-5958. © 2020 Wiley-VCH GmbH.²²⁸