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. Author manuscript; available in PMC: 2021 Sep 4.
Published in final edited form as: Int Immunopharmacol. 2020 May 22;85:106612. doi: 10.1016/j.intimp.2020.106612

Figure 1. iNOS expression was upregulated in human OA cartilage and chondrocytes, and IL-1β-induced the expression of iNOS in OA chondrocytes.

Figure 1.

(A) Safranin O/Fast green staining to visiualize damaged (superficial) and undamaged (deep zone) area of OA cartilage (n=5) (20X magnification). (B) Expression of iNOS in damaged and undamaged region of OA cartilage determined by IHC (20X magnification) and (C) Quantification of the IHC data. (D) iNOS expression at mRNA level in damaged and undamaged human OA cartilage (n=5). β-Actin was used as a normalization control. (E) iNOS expression in chondrocytes treated with IL-1β at mRNA level and (F) protein level. β-actin was used as endogenous normalization or loading control. (G) Culture supernatant was used to determine the level of NO production. (*p≤0.05, **p ≤0.005).