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. Author manuscript; available in PMC: 2021 Sep 4.
Published in final edited form as: Int Immunopharmacol. 2020 May 22;85:106612. doi: 10.1016/j.intimp.2020.106612

Figure 2. Imperatorin suppressed IL-1β-induced iNOS expression and production of NO in OA chondrocytes.

Figure 2.

(A) Effect of Imperatorin (50, 25 & 10 μM) on chondrocyte viability was determined by MTT assay. Chondrocytes treated with 0.1% DMSO served as control. (B) iNOS mRNA expression in OA chondrocytes treated with IL-1β in the presence or absence of Imperatorin. β-actin was used as endogenous expression control (* p≤0.05). (C) Expression of iNOS at protein level in OA chondrocytes treated with IL-1β in the presence or absence of Imperatorin. (D) Quantification of band intensity by ImageJ. (E) Chondrocyte culture supernatant from the above experiment was used to NO levels. The values are mean ± SD of five independent experiments (**p ≤0.005). The values are mean ± SD of three independent experiments.