Figure 4.

Disruption of orf352 partially restores pollen development but does not restore pollen fertility. A–G, Pollen grain viability in the various recombinant types, as determined by staining with Lugol’s iodine (1% I₂-KI). H–N, Pollen germination and pollen tube elongation on stigmas, observed by staining pollen tubes with aniline blue. O, Percentage of darkly stained pollen grains (viable) in each recombinant type. Scale bars = 200 μm (A–G) and 100 μm (H–N). The data are representative of six independent experiments.