Figure 6.

The function of the PtDAL1 and PtMADS11 in the flowering regulatory network in Arabidopsis and pine. Transgenic phenotypes of 35S::DAL1 in wild-type and loss-of-function mutant Arabidopsis. a, Ectopic expression of the PtDAL1 gene significantly advanced the flowering of wild-type Arabidopsis; b, overexpression of PtDAL1 rescued the late flowering of 35S::miR156A Arabidopsis lines; c, the late-flowering phenotype of the spl1,2,3,4,5,6 mutant was restored by overexpression of PtDAL1; d, the early flowering phenotype of the PtDAL1 overexpression lines was suppressed by ft-10 mutation; e, the overexpression of 35S::PtDAL1 in soc1-1-2 mutant. f, 35S::PtMADS11 transgenic Arabidopsis exhibited early flowering under SD conditions; g, heterotopic expression of PtMADS11 restored the severe late-flowering phenotype of 35S::miR156A Arabidopsis; h, overexpression of PtMADS11 accelerated flowering with ft-10 mutation; i, transgenic PtMADS11 in the soc1-1-2 mutant caused no apparent alteration in morphology; and j, no obvious phenotypes of overexpression of 35S::MADS11 in wild-type Arabidopsis under LD conditions. The parts of the figure were assembled from more than one photo, and the outlines of each original photo are indicated by dotted lines. B, The model of the PtDAL1 and PtMADS11 in the flowering regulatory network of Arabidopsis. *, under SD conditions. C, The expression patterns of PtDAL1 and PtMADS11 in P. tabuliformis growth process. The red and blue indicate high- and low-expression level, respectively. PtDAL1 was expressed at low levels in seedlings, and gradually accumulated with age (black triangle). And PtMADS11 was suddenly activated in tree at 7 years, and then the expression increased with age (green quadrilateral). In adult trees, PtMADS11 had a downward trend of top-down expression, while PtDAL1 is relatively consistently expressed between different layer branches. The cones in the trees indicate the reproductive ability of these trees.