Figure 1.
The fhy3 mutants have seed dormancy and germination responses. A, Diagram of light treatments. Seeds were sown on plates under white light (WL) within 1 h and then exposed to far-red (FR) light for 5 min followed by a subsequent 5-min red (R) light pulse (phyB-on) or not (phyB-off). Seeds were incubated in darkness at 22°C for 4 d before recording germination frequency. B and D, Germination phenotype. C and E, Germination frequency of fhy3 mutants and the corresponding wild-type as shown in B and D, respectively. F and G, Germination frequency under a 16-h white light and 8-h dark photoperiodic condition. For B–G, seeds were harvested and stored at room temperature for 9 months and germination was performed without cold stratification. H and I, Germination frequency of freshly harvested seeds without cold treatment. J and K, Germination frequency of freshly harvested seeds after 3 d of 4°C cold treatment. For C, E–K, data are means ± SD, n=3 biological repeats.