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. 2021 Mar 25;187(1):289–302. doi: 10.1093/plphys/kiab147

Figure 3.

Figure 3

FHY3 regulates downstream gene expression. A, Venn diagram showing the overlap of differentially regulated genes in fhy3-11 compared to Col wild-type. Freshly harvested seeds were imbibed under white light for 24 h before RNA extraction. B, GO analysis of commonly regulated genes by FHY3. C, List of partial FHY3-regulated genes from RNA-seq. D, RT-qPCR analysis of transcript accumulation in Col wild-type, fhy3-11, and phyB-9 mutants. Freshly harvested seeds were imbibed under white light or in darkness for 24 h. E, RT-qPCR analysis of gene expression in Col and fhy3-11 under phyB-on and phyB-off conditions. After-ripened seeds were imbibed for 24 h. For (D) and (E), data are means ± SD, n=3 biological replicates. Relative expression was normalized to the expression level of Actin2.