Figure 5.
Genetic interaction between FHY3 and RVE2/RVE7 and GA3ox2. A, Germination phenotype of Col wild-type and various mutants. Freshly harvested seeds were germinated under white light condition for 4 d. B, Germination frequency of seeds as shown in (A). C, Germination phenotype of Col wild-type and various mutants. After-ripened seeds were germinated under phyB-on conditions. D, Germination frequency of seeds as shown in (C). E, Germination frequency of fhy3, GA3ox2-OX, and their double mutants. F and G, GA3ox2 expression in Col versus fhy3-11 mutant in freshly harvested seeds during imbibition for up to 24 h under white light (F) or in post-harvested seeds after imbibition for 24 h under phyB-on condition (G). For (B), (D–G), data are means ± SD, n=3 replicates. Relative expression was normalized to the expression level of Actin2.