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. 2021 Jun 7;187(1):203–217. doi: 10.1093/plphys/kiab261

Figure 4.

Figure 4

SHLF represents a repeat-containing novel and conserved bryophyte-specific gene family. A, The SHLF (Pp3c14_22870) gene structure and the Tnt1 (∼5.6 kb) insertion site are depicted. In both the genome and mRNA, the SHLF gene contains four TDRs each 513 bp long that translates into a protein with four TDRs, each 171 aa long. B, Tissue-specific expression of SHLF in WT protonema, gametophore, and gametophore apex (Ct value for α-ACTIN and SHLF is 21 and 16, respectively). The error bars represent standard deviation. C and D, The percentage of sequence similarity among the four TDRs at nucleotide level (C) and amino acid level (D) is shown as domain-dot plot and multiple sequence alignment, respectively. E, The presence and absence of SHLF orthologs among the sequenced genomes of all green lineages (blue colored species name indicates presence of SHLF ortholog/s). The near-perfect TDRs in both gene and protein sequences are a unique feature of this bryophyte-specific SHLF gene family.