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. 2021 Jun 12;187(1):59–72. doi: 10.1093/plphys/kiab272

Figure 1.

Figure 1

Enrichment of cell type-specific markers in BS and M fractions. A, Immunoblots showing abundance of marker proteins in BS and M fractions. Replicate blots were probed with the indicated antibodies. BS markers: NADP-dependent malic enzyme (NADP-ME), H subunit of the NDH-like complex (NdhH), Rubisco large subunit (RbcL). M markers: phosphoenolpyruvate carboxylase (PEPC), pyruvate orthophosphate dikinase (PPDK), D2 subunit of Photosystem II (D2). An image of one blot stained with Ponceau S is shown below. B, Abundance of RNA-seq and ribo-seq reads from BS and M marker genes. BS markers: NADP-ME (Zm00001d000316) and Rubisco small subunit (RBCS; Zm00001d052595). M markers: PEPC (Zm00001d046170) and PPDK (Zm00001d038163). RNA-seq data from Tausta et al. (2014) (leaf section 14 with 2 replicates) and Denton et al. (2017) (leaf section S1 with three replicates) are compared with our data. Each line symbol represents normalized reads for one gene (see color key at top) in one replicate. RPKM, reads per kilobase per million reads mapped to nuclear coding sequences. C, Abundance of RNA-seq and ribo-seq reads from epidermis and phloem marker genes. Data for additional marker genes are shown in Supplemental Figure S2C.