Figure 5.

OsFes1C directly interacts with OsBiP1. A, CoIP assay of transgenic OsFes1C-FLAG and endogenous interacting proteins using transgenic calli. All the bands were identified by mass spectrometric analyses, respectively. Two proteins, OsBiP1 and OsSalt, were immunoprecipitated with OsFes1C. The asterisk indicates the degraded bands of OsFes1C-FLAG. B, The GST pull-down assay shows that the OsFes1C-His was pulled down only with GST-OsBiP1, but not with the GST control. C, The interaction between OsFes1C and OsBiP1 in plant cells was confirmed by BiFC. The scale bar (50 μm) for the top left image apply to all images. D, Firefly LCI assay for interaction between cLUC-OsFes1C and OsBiP1-nLUC in N. benthamiana. E, ADP strengthens the interaction between OsFes1C and OsBiP1, and ATP weakens the interaction. The ratios of OsFes1C-His to GST-OsBiP1 in each condition were shown. The asterisks indicate the degraded bands of GST-OsBiP1.