Figure 2.

Normal SPA2 destabilization depends on COP10 and DET1. A and C, SPA2 protein levels in WT and cop10 (A) and det1-1 (C) mutant seedlings that were grown in darkness for 4 d and then transferred to far-red light (FR) (0.35 µmol m⁻² s⁻¹) for the indicated time. SPA2 was detected in nuclear-enriched extracts using α-SPA2 antibodies. Histone H3 (H3) detected by specific antibodies was used as loading control. B and D, Quantification of relative SPA2/H3 protein levels. SPA2/H3 levels in the dark-grown WT was set to 1. Error bars indicate the SEM of two independent experiments.