FIGURE 1.

Variation in cycle thresholds for high and low copy number genes at different concentrations. Figure 1 shows the results of using 36B4 as an internal reference gene showed that the △CT values (△CT refers to the difference between the measured CT value of the telomere and the CT value of the internal reference gene) of different concentrations of the same sample were slightly different. In comparison, when YH‐1 was used as an internal reference gene, the △CT values of different samples completely overlapped indicating that YH‐1 was more stable as an internal reference gene