FIGURE 2.

ASPM silence suppresses LIHC cells growth and metastasis. For functional experiments, SNU449 and Hepg3b cells were stably infected with ASPM shRNA, and the transfection efficacy was detected by Western blotting (A) and qPCR (B). CCK‐8 (C) and colony formation (D) were used to evaluate the proliferation rate. The transwell invasion (E) and wound scratch and (F) assays were adopted to examine the migratory and invasive capacity. G. Western blotting was utilized to detect EMT‐related proteins.*p < 0.05, **p < 0.01, ***p < 0.001. Data represent at least three independent sets of experiment