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. 2021 Aug 16;35(9):e23931. doi: 10.1002/jcla.23931

FIGURE 3.

FIGURE 3

ASPM overexpression promotes LIHC cells growth and metastasis. For further investigation, MHCC‐97H and Hepg2 cells were infected with ASPM overexpression plasmid, and the transfection efficacy was detected by Western blotting (A) and qPCR (B). CCK‐8 (C) and colony formation (D) were used to measure the proliferation rate. The transwell invasion (E) and wound scratch (F) assays were used to examine the invasion and migration. G. Western blotting was employed to detect EMT‐related proteins. **p < 0.01, ***p < 0.001. Data represent at least three independent sets of experiment