FIGURE 4.

METTL3 mediated m6A modification of ASPM in LIHC. (A) SRAMP database was performed to predict m6A sequences motif of METTL3 and ASPM. (B) STARbase database was performed to analyze the correlation between the expression of METTL3 and ASPM. For further confirmation, SNU449 cells were stably infected with METTL3 knockdown, and ASPM protein and mRNA expression level were measured by qPCR (C) and Western blotting (D). (E) ASPM was silenced in LIHC cells and MeRIP‐qPCR was adopted to test m6A levels of ASPM. (F) ASPM was overexpressed in LIHC cells and MeRIP‐qPCR was adopted to test m6A levels of ASPM. (G) MeRIP‐PCR was utilized to assess the m6A levels of ASPM when METTL3 was knockdown. **p < 0.01, ***p < 0.001. Data represent at least three independent sets of experiment