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. 2021 Aug 17;35(9):e23952. doi: 10.1002/jcla.23952

FIGURE 5.

FIGURE 5

ERRB3 directly targets miR‐22‐3p in BRCA. (A) A Venn diagram showed that 68 genes may be functional targets of miR‐22‐3p. (B) The expression of AKT3, PTEN, ESR1, P53, and ERBB3 in MCF7 cells detected by RT‐qPCR after the transfection of miR‐22‐3p mimics. (C) Correlation analysis between ERBB3 and miR‐22‐3p based on starBase database. (D) The expression of ERBB3 protein in MCF7 cells detected by WB after the transfection of miR‐22‐3p inhibitor or mimics. (E) Ago2‐RIP assay assessed endogenous Ago2 binding to ERBB3 and miR‐22‐3p in MCF7 cells. (F) The binding sites between ERBB3 and miR‐22‐3p predicted by TargetScan. (G) Dual‐luciferase assay confirmed the direct interaction between ERBB3 and miR‐22‐3p. Note: *p < 0.05, **p < 0.01, and ***p < 0.005