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. 2021 Sep 4;7:230. doi: 10.1038/s41420-021-00620-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Confocal microscope images of calcium signaling (green) in cultured primary neurons using Fluo-8 AM in control, Aβ + PBS, Aβ + EVs groups (bar = 50 μm). B Igor software assay shows the two phases (20 and 50 seconds, respectively) of the intracellular calcium transients in each group. C–E Statistical analysis data of the amplitude (ΔF/F) (C), rise time (D), and decay time (E) of the calcium signaling after adding ATP in each group (n > 70 cells per group). Data expressed as scatter plots with Mean ± SEM. The data meet normal distribution and the variance is homogeneous. *P < 0.05, ****P < 0.0001.