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. 2021 Sep 4;6:333. doi: 10.1038/s41392-021-00702-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — CEBPA drives cellular differentiation of NPC-derived cells. a Protein levels of CEBPA in various NPC-derived cell lines with different differentiation status. b RT-qPCR analysis of relative CEBPA mRNA levels in various NPC-derived cell lines with different differentiation status. c Western blot with differentiation and stem-like markers in control (Vector) and CEBPA-overexpressed (CEBPA) CNE2 cells. d Immunofluorescence analysis with differentiation markers in control and CEBPA-overexpressed CNE2 cells. e Colony formation in control and CEBPA-overexpressed CNE2 cells. f Immunofluorescence staining for Ki67 in control and CEBPA-overexpressed CNE2 cells. g SA-β-gal staining in control and CEBPA-overexpressed CNE2 cells. h CEBPA-overexpressed and control CNE2 cells were injected into nude mice subcutaneously, and tumor volume was determined. i RT-qPCR analysis of relative CEBPA mRNA levels in patients’ tumor tissues non-tumor nasopharyngeal tissues. Statistics (d–i), significance: *P < 0.05, **P < 0.01, ***P < 0.001; two-tailed Student’s t-tests