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. 2021 Sep 4;6:333. doi: 10.1038/s41392-021-00702-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — CEBPA overcomes LMP1-induced dedifferentiation in NPC cells. a Western blot analysis with differentiation and stem-like markers in CNE1-TetOn-LMP1 and CEBPA-overexpressed CNE1-TetOn-LMP1 cells by 100 ng/ml Dox treatment for 48 hours. b Proliferation ability of CNE1-TetOn-LMP1 and CEBPA-overexpressed CNE1-TetOn-LMP1 cells was determined by CCK8 assay. c Colony formation in CNE1-TetOn-LMP1 and CEBPA-overexpressed CNE1-TetOn-LMP1 cells. d Immunofluorescence staining for Ki67 in CNE1-TetOn-LMP1 and CEBPA-overexpressed CNE1-TetOn-LMP1 cells. e SA-β-gal staining in CNE1-TetOn-LMP1 and CEBPA-overexpressed CNE1-TetOn-LMP1 cells. f CNE1-TetOn-LMP1 and CEBPA-overexpressed CNE1-TetOn-LMP1 cells were injected into nude mice subcutaneously. After continuous Dox administration, tumor volume was determined. g CEBPA, differentiation, and stem-like markers were analyzed by immunoblot in tumor tissue and primary cultured tumor cells. h IHC staining of differentiation markers in CNE1-TetOn-LMP1 and CEBPA-overexpressed CNE1-TetOn-LMP1 tumor tissues. Representative images are shown. Statistics (b–f), significance: **P < 0.01, ***P < 0.001; two-tailed Student’s t-test