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. Author manuscript; available in PMC: 2021 Sep 5.
Published in final edited form as: Cell Rep. 2021 Aug 24;36(8):109605. doi: 10.1016/j.celrep.2021.109605

Figure 5. Chemogenetic inhibition of the PVT→NAc pathway reduces heroin relapse.

Figure 5.

(A) Schematic illustration of the combinatorial virus approach employed in our study.

(B) Placement map showing hM4Di-mCherry expression in the PVT (each animal represented at 8% opacity).

(C and D) Chemogenetic inhibition of the PVT→NAc pathway with the DREADD agonist J60 reduced cocaine-induced Fos expression in hM4Di-mCherry-expressing neurons (vehicle [Veh], n = 10; J60, n = 12).

(E) Timeline employed for the behavioral experiment.

(F and G) Chemogenetic inhibition of the PVT→NAc pathway reduces heroin relapse after abstinence (F; n = 11) but not extinction training (G; n = 12) from heroin self-administration. *p < 0.05, **p < 0.01 versus vehicle.

Scale bars, 50 μm. Data are shown as mean ± SEM.