Table 1.
Results of complete blood count changes in rabbits on the day before injection (T0) and 2 weeks after the last injection (T1) as mean±standard deviation and 95% CI in pentavalent toxoid clostridial vaccination with and without nanoparticles of chitosan and control groups
| Factor | P-value | Without chitosan | With chitosan | Control | Mean | Time |
|---|---|---|---|---|---|---|
| White blood cells (×103/µl) | 0.06 | 7.76±0.70 | 7.83±1.38 | 7.43±0.60 | Mean±standard deviation | T0 |
| 7.25-8.26 | 6.83-8.82 | 6.99-7.86 | 95% CI | |||
| 0.005 | 7.88±1.91ab | 8.80±1.78b | 6.67±1.98ab | Mean±standard deviation | T1 | |
| 6.65-9.59 | 7.52-10.07 | 5.15-8.20 | 95% CI | |||
| - | 0.74 | 0.12 | 0.39 | *P-value | ||
| b, ab Significant increase in mean of white blood cells 35 days after vaccination accompanied with chitosan; *P<0.05 | ||||||
| Red blood cells (×106/µl) | 0.28 | 5.75±0.50 | 5.37±0.38 | 5.29±0.79 | Mean±standard deviation | T0 |
| 5.39-6.11 | 5.09-5.64 | 4.72-5.86 | 95% CI | |||
| 0.23 | 5.42±0.81 | 5.10±0.59 | 5.61±1.09 | Mean±standard deviation | T1 | |
| 4.79-6.04 | 4.68-5.53 | 4.77-6.46 | 95% CI | |||
| 0.3 | 0.16 | 0.66 | *P-value | |||
| No significant change; *P<0.05 | ||||||
| Hemoglobin (gm/dl) | 0.61 | 13.13±0.66 | 12.56±1.001 | 12.11±1.30 | Mean±standard deviation | T0 |
| 12.65-13.6 | 11.84-13.27 | 11.18-13.04 | 95% CI | |||
| 0.26 | 12.44±2.24 | 12.28±1.38 | 12.14±1.91 | Mean±standard deviation | T1 | |
| 10.72-14.16 | 11.29-13.26 | 10.67-13.61 | 95% CI | |||
| - | 0.40 | 0.52 | 0.88 | *P-value | ||
| No significant change; *P<0.05 | ||||||
| Packed cell volume (%) | 0.62 | 41.50±3.09 | 40.17±2.60 | 38.53±2.27 | Mean±standard deviation | T0 |
| 39.28-43.71 | 38.30-42.03 | 36.90-40.15 | 95% CI | |||
| 0.12 | 40.18±6.48 | 40.10±3.23 | 39.80±5.76 | Mean±standard deviation | T1 | |
| 35.20-45.17 | 37.78-42.41 | 34.59-45 | 95% CI | |||
| - | 0.48 | 0.93 | 0.68 | *P-value | ||
| No significant change; *P<0.05 | ||||||
| Platelet (×µl.103) | 0.10 | 429.60±49.46 | 489.10±51.77 | 442.30±65.55 | Mean±standard deviation | T0 |
| 394.21-464.98 | 452.06-526.13 | 395.40-489.19 | 95% CI | |||
| 0.03 | 394.55±139.50ab | 504.80±150.86b | 350.77±90.78a | Mean±standard deviation | T1 | |
| 287.32-501.78 | 396.87-612.72 | 280.99-420.56 | 95% CI | |||
| - | 0.62 | 0.78 | 0.10 | *P-value | ||
| a, b, ab Significant increase in mean of platelet 35 days after vaccination; *P<0.05 | ||||||
| Heterophile | 0.56 | 70.45±5.20 | 98.20± 4.44 | 62.70- 4.45 | Mean±standard deviation | T0 |
| 97.41-49.42 | 76.63-47.40 | 39.005-49.42 | 95% CI | |||
| 0.0001 | 40.24±4.58bc | 81.70±5.37c | 87.00±1.150a | Mean±standard deviation | T1 | |
| 91.43-96.43 | 85.54-41.33 | 43.56-51.48 | 95% CI | |||
| 0.06 | 0.008 | 0.04 | *P-value | |||
| a, b, ab Significant decrease in mean of heterophile 35 days after vaccination; *P<0.05 | ||||||
| Lymphocyte | 0.25 | 50.50±4.48 | 39.80±4.50 | 82.20±3.48 | Mean±standard deviation | T0 |
| 72.27-51.45 | 94.65-53.47 | 93.46-50.45 | 95% CI | |||
| 0.0001 | 86.22±3.50ab | 69.10±6.56 b | 20.1±2.44 a | Mean±standard deviation | T1 | |
| 19.25-53.47 | 88.31-60.51 | 66.55-45.42 | 95% CI | |||
| 0.35 | 0.055 | 0.53 | *P-value | |||
| a, b, ab Significant increase in mean of lymphocyte 35 days after vaccination; *P<0.05 | ||||||
| Eosinophil | 0.62 | 50.00±1.2 | 31.80±1.1 | 97.5±0.2 | Mean±standard deviation | T0 |
| 75.24-2.1 | 74.85-2.0 | 19.80-3.1 | 95% CI | |||
| 0.31 | 10.88±1.1 | 8.10±0.1 | 79.33±0.1 | Mean±standard deviation | T1 | |
| 70.70-3.0 | 88.31-1.0 | 10.65-2.0 | 95% CI | |||
| 0.87 | 0.32 | 0.01 | *P-value | |||
| Significant decrease of eosinophil in control group;*P<0.05 | ||||||
| Basophile | 0.85 | 60.07±1.1 | 30.20±1.1 | 97.50±0.1 | Mean±standard deviation | T0 |
| 83.36-0.2 | 93.46-1.03 | 19.80-20 | 95% CI | |||
| 0.01 | 95.33±0.1ab | 45.90±0.0 b | 33.44±1.2 a | Mean±standard deviation | T1 | |
| 0.2-19.47 | 68.11-1.0 | 46.41-3.1 | 95% CI | |||
| 0.83 | 0.43 | 0.15 | *P-value | |||
| a, b, ab Significant difference in mean of basophile after vaccination; *P<0.05 | ||||||
| Monocyte | 0.98 | 13.20±1.2 | 24.00±1.2 | 10.10±1.2 | Mean±standard deviation | T0 |
| 3.01-3.1 | 81.1-2.1 | 88.31-2.1 | 95% CI | |||
| 0.20 | 72.11±3.6b | 20.57± 2.04ab | 26.1±11.2a | Mean±standard deviation | T1 | |
| 32.89-3.8 | 2.6-35.04 | 3.1-13/08 | 95% CI | |||
| 0.006 | 0.04 | 0.84 | *P-value | |||
| a, b, ab Significant difference in monocyte after vaccination; *P<0.05 | ||||||
| Neut/Lymph | 0.36 | 0.95±0.19 | 0.88±0.17 | 0.95±0.16 | Mean±standard deviation | T0 |
| 0.81-1.09 | 0.75-1.008 | 0.84-1.07 | 95% CI | |||
| 0.0001 | 0.81±0.12 | 0.69±0.18 | 1.13±0.08 | Mean±standard deviation | T1 | |
| 0.71-0.90 | 0.56-0.82 | 0.07-1.19 | 95% CI | |||
| 0.11 | 0.02 | 0.04 | *P-value | |||
| Significant decrease of Neut/Lymph after vaccination; *P<0.05 | ||||||