KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| mouse anti-S100β | Sigma-Aldrich | Cat# S2532; RRID:AB_477499 |
| rabbit anti-S100β | Abcam | Cat# ab41548; RRID: AB_956280 |
| mouse anti-NeuN (clone A60) | Millipore | Cat# MAB377; RRID: AB_2298772 |
| chicken anti-GFP | Abcam | Cat# ab13970; RRID: AB_300798 |
| rabbit anti-RFP | Rockland | Cat# 600–401-379; RRID: AB_2209751 |
| rabbit anti-c-Fos | Millipore | Cat# ABE457; RRID: AB_2631318 |
| Alexa Fluor 488 goat anti-chicken | Molecular Probes | Cat# A11039; RRID: AB_2534096 |
| Alexa Fluor 488 goat anti-rabbit | Molecular Probes | Cat# A11008; RRID: AB_143165 |
| Alexa Fluor 546 goat anti-mouse | Molecular Probes | Cat# A11003; RRID: AB_2534071 |
| Alexa Fluor 546 goat anti-chicken | Molecular Probes | Cat# A11040; RRID: AB_2534097 |
| Alexa Fluor 594 goat anti-rabbit | Molecular Probes | Cat# R37117; RRID: AB_2556545 |
| Alexa Fluor 647 goat anti-rabbit | Molecular Probes | Cat# A21245; RRID: AB_ 2535812 |
| Bacterial and Virus Strains | ||
| pcDNA 3.1 CMV-iβARK-p2A-mCherry | This paper | Addgene Vectors #117689 |
| pcDNA 3.1 CMV-iβARK(D110A)-p2A-mCherry | This paper | Addgene Vectors #117690 |
| CMV-iβARK(R106A)-p2A-mCherry | This paper | Addgene Vectors #117688 |
| AAV2/5 GfaABC1D-iβARK-p2A-mCherry | This paper | Addgene Vectors #117691 |
| AAV2/5 GfaABC1D-iβARK(D110A)-p2A-mCherry | This paper | Addgene Vectors #117692 |
| PHP.eB AAV CAG-FLEx-iβARK-p2A-mCherry | This paper | Addgene Vectors #117693 |
| PHP.eB AAV CAG-FLEx-iβARK(D110A)-p2A-mCherry | This paper | Addgene Vectors #117694 |
| AAV2/5 GfaABC1D cyto-GCaMP6f | Chai et al., 2017 | Addgene Vectors #52925-AAV5 RRID:Addgene_52925 |
| AAV2/5 GfaABC1D Rpl22HA | Yu et al., 2018 | Addgene Vectors #111811 RRID:Addgene_111811 |
| AAV5 GfaABC1D-mCherry-hPMCA2w/b | Yu et al., 2018 | Addgene Vectors #111568 RRID:Addgene_111568 |
| AAV2/5 GfaABC1D hM3Dq-mCherry | Chai et al., 2017 | Addgene Vectors #92284 RRID:Addgene_92284 |
| AAV2/5 GfaABC1D hM4Di-mCherry | Chai et al., 2017 | Addgene Vectors #92286 RRID:Addgene_92286 |
| AAV2/5 GfaABC1D rM3Ds-mCherry | Chai et al., 2017 | Addgene Vectors #92285 RRID:Addgene_92285 |
| AAV2/5 GfaABC1D Lck-GFP | Shigetomi et al., 2013 | Addgene Vectors #105598-AAV5 RRID:Addgene_105598 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Formalin, Buffered, 10% | Fisher Chemical | Cat# SF100–20 |
| Pronase | Sigma-Aldrich | Cat# P6911 |
| Fetal bovine serum | Thermo Fisher Scientific | Cat# 10437028 |
| BSA | Sigma-Aldrich | Cat# A8806 |
| Actinomycin D | Sigma-Aldrich | Cat# A1410 |
| TTX | Cayman Chemical | Cat# 14964 |
| R-baclofen | Tocris | Cat#0796 |
| Clozapine N-oxide (CNO) | Tocris | Cat#4936 |
| ATP | Tocirs | Cat#3245 |
| Adenosine | Tocirs | Cat#3624 |
| Phenylephrine | Tocris | Cat#2838 |
| Tamoxifen | Sigma-Aldrich | Cat#T5648 |
| Experimental Models: Organisms/Strains | ||
| Mouse: Itpr2tm1.1Chen | Li et al., 2005 (gift from Martin Paukert at UT San Antonio) | RRID:MGI:3641042 |
| Mouse: B6;FVB-Tg(Aldh1l1-cre/ERT2)1Khakh/J | Srinivasan et al., 2016 | RRID:MGI:5806568 |
| Mouse: C57Bl/6NJ | Jackson Laboratory | RRID: IMSR_JAX:005304 |
| Software and Algorithms | ||
| OriginPro 2016 | Origin Lab Corporation | RRID:SCR_015636 https://www.originlab.com/origin |
| pCLAMP10.4 | Molecular Devices | RRID:SCR_011323 https://www.moleculardevices.com |
| ClampFit10.4 | Molecular Devices | N/A https://www.moleculardevices.com |
| Fluoview FV10-ASW | Olympus | N/A https://www.olympus-lifescience.com/ |
| ImageJ v1.51h | NIH | RRID:SCR_003070 https://imagej.nih.gov/ij/download.html |
| CorelDraw X7 | Corel Corporation | RRID:SCR_014235 https://www.coreldraw.com |
| Labview 2011 | National Instruments | RRID:SCR_014325 https://www.ni.com/ |
| Bioconductor | Bioconductor | http://www.bionconductor.org |
| GraphPad Prism | GraphPad Software LLC | RRID:SCR_002798 https://www.graphpad.com |
| Deposited Data | ||
| Bulk tissue and astrocyte RNA-seq: iβARK vs iβARK(D110A) | This paper | GEO: GSE158876 |
| Statistical tests and results | Excel File S1 | N/A |