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. Author manuscript; available in PMC: 2022 Sep 2.
Published in final edited form as: Structure. 2021 Mar 12;29(9):940–950.e4. doi: 10.1016/j.str.2021.02.006

Figure 3. ITC and SPR analysis of GW1929 binding to PPARγ LBD.

Figure 3.

(A) Heat capacity (ΔCp) analysis from temperature-dependent ITC titrations of GW1929 into PPARγ LBD. Data are represented as a fitted ΔH and ΔG values from the ITC analysis (n=1). (B) SPR analysis of GW1929 binding to PPARγ LBD fit with a two-step binding equation accounting for a conformational change after binding model. (C) A two-state induced fit binding model consistent with the NMR CSP titration, ITC, and SPR data with the SPR rate constants shown from the fit of the data in (B). See also Figure S2.