| Antibodies |
| LanthaScreen Elite Tb-anti-His Antibody |
Thermo Fisher Scientific |
Cat: PV5863 |
| Bacterial and Virus Strains |
| Escherichia coli BL21(DE3) cells |
Invitrogen |
Cat: C600003 |
| Chemicals, Peptides, and Recombinant Proteins |
| Ammonium Chloride, 99%15N |
Cambridge Isotope Labs |
Cat: NLM-467 |
| Deuterium Oxide, 99.9% D |
Sigma-Aldrich |
Cat: 151882 |
| Dimethyl Sulfoxide-d6, 99.9% D |
Cambridge Isotope Labs |
Cat: DLM-10 |
| FITC-NH-NTKNHPMLMNLLKDNPAQD |
LifeTein, LLC |
N/A |
| HiLoad 16/600 Superdex 75 pg column |
GE Healthcare Life Sciences |
Cat: 28989333 |
| HisTrap FF column |
GE Healthcare Life Sciences |
Cat: 17525501 |
| NTA Reagent Kit |
GE Healthcare Life Sciences |
Cat: 28995043 |
| HBS-P+ Buffer |
GE Healthcare Life Sciences |
Cat: BR100827 |
| Isopropyl-β-D-thiogalactoside |
Gold Biotechnology |
Cat: I2481C |
| TCEP (Tris (2-Carboxyethyl) phosphine Hydrochloride) |
Gold Biotechnology |
Cat: TCEP25 |
| Sodium citrate tribasic dihydrate |
Hampton Research |
Cat: HR2–549 |
| MOPS sodium salt |
Sigma-Aldrich |
Cat: M9381 |
| Imidazole |
Sigma-Aldrich |
Cat: 792527 |
| Potassium Chloride |
Sigma-Aldrich |
Cat: 746436 |
| Potassium phosphate monobasic |
Sigma-Aldrich |
Cat: 795488 |
| Potassium phosphate dibasic |
Sigma-Aldrich |
Cat: P3786 |
| Trizma Base |
Sigma-Aldrich |
Cat: 93352 |
| Ethylenediaminetetraacetic acid disodium dihydrate |
Sigma-Aldrich |
Cat: E9884 |
| Sodium Chloride |
Sigma-Aldrich |
Cat: 746398 |
| Sodium phosphate dibasic |
Sigma-Aldrich |
Cat: S5136 |
| Calcium chloride dihydrate |
Sigma-Aldrich |
Cat: 223506 |
| Manganese (II) chloride tetrahydrate |
Sigma-Aldrich |
Cat: 221279 |
| Copper (II) Chloride Dihydrate |
Sigma-Aldrich |
Cat: 22201–1 |
| Tryptone |
Fisher Scientific |
Cat: BP1421–2 |
| Yeast Extract |
Fisher Scientific |
Cat: BP1422–2 |
| D-Glucose |
Fisher Scientific |
Cat: FLBP350–1 |
| Magnesium sulfate |
Fisher Scientific |
Cat: BP213–1 |
| Ampicillin |
Fisher Scientific |
Cat: BP1760 |
| Ferrous sulfate heptahydrate |
Fisher Scientific |
Cat: I146–500 |
| Cobalt (II) chloride hexahydrate |
Acros Organic |
Cat: 7791–13-1 |
| Zinc sulfate heptahydrate |
EMD Millipore |
Cat: ZX0065–1 |
| Fluormone Pan-PPAR Green |
Invitrogen |
Cat: PV4894 |
| Darglitazone |
Cayman Chemical |
Cat: 17681 |
| GW1929 |
Cayman Chemical |
Cat: 13689 |
| MRL24 |
BioVision |
Cat: 2764–5 |
| Human PPARγ LBD WT, residues 203–477; isoform 1 protein |
This paper |
N/A |
| Human PPARγ LBD Y473E mutant protein |
This paper |
N/A |
| Deposited Data |
| Human PPARγ LBD in Complex with Darglitazone crystal structure |
(Shang et al., 2019) |
PDB: 6DGL |
| Human PPARγ LBD-Apo Delipidated crystal structure |
This paper |
PDB: 6VZO |
| Human PPARγ LBD in Complex with GW1929 crystal structure |
This paper |
PDB: 6VZL |
| Human PPARγ LBD-Y473E crystal structure |
This paper |
PDB: 6VZN |
| Human PPARγ LBD-Y473E in Complex with Darglitazone crystal structure |
This paper |
PDB: 6VZM |
| Human PPARγ LBD-Y473E in Complex with GW1929 crystal structure |
This paper |
PDB: 7JQG |
| Human PPARγ LBD Bound to GW1929 NMR chemical shift assignments |
(Lu et al., 2008) |
BMRB: 15518 |
| Oligonucleotides |
| Y473E mutagenesis forward primer: 5’-CTGCAGGAGATCGAAAAGGACTTGTAC-3’ |
IDT technologies |
N/A |
| Y473E mutagenesis reverse primer: 5’-GTACAAGTCCTTTTCGATCTCCTGCAG-3’ |
IDT technologies |
N/A |
| Recombinant DNA |
| Plasmid: pET-46 Ek/LIC |
Novagen |
Cat#71335 |
| pET46-PPARγ LBD WT |
(Hughes et al., 2012) |
N/A |
| pET46-PPARγ LBD Y473E |
This paper |
N/A |
| Software and Algorithms |
|
|
| NMRViewJ |
OneMoon Scientific, Inc. (Johnson 2018) |
https://nmrfx.org/nmrfx/nmrviewj
|
| NMRFx Analyst |
OneMoon Scientific, Inc. (Norris et al. 2016; Johnson 2018) |
https://nmrfx.org/nmrfx/analyst
|
| Topspin |
Bruker Biospin |
https://www.bruker.com/products/mr/nmr/software/topspin.html
|
| GraphPad Prism |
GraphPad Software Inc. |
http://www.graphpad.com/scientific-software/prism/
|
| PyMOL |
Schrödinger |
https://pymol.org/2/
|
| PHENIX |
(Adams et al., 2011) |
http://www.phenix-online.org/
|
| CCP4 |
(Winn et al., 2011) |
http://www.ccp4.ac.uk/
|
| COOT |
(Emsley et al., 2004) |
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
|
| iTC200 software |
MicroCal Inc. |
v 1.24.2 |
| NITPIC |
(Keller et al., 2012) |
http://biophysics.swmed.edu/MBR/software.htm
|
| GUSSI |
(Brautigam et al., 2016) |
http://biophysics.swmed.edu/MBR/software.htm
|
| SEDPHAT |
(Houtman et al., 2007) |
http://www.analyticalultracentrifugation.com/sedphat/
|
