Figure 1.

Increases of PRMT5 expression correlated with poor prognosis in cervical cancer. (A) PRMT5 expression and SDMA levels in a normal human cervical cell line and four human cervical cancer cell lines were analyzed using western blot (left panel), and the protein expression of PRMT5 was quantified by ImageJ (right panel). Values are presented as the mean ± SEM. (B) Violin plot showed PRMT5 expression of normal cervical tissues (n = 13) and cervical tumor tissues (n = 306) from TCGA database (Kruskal-Wallis test). (C) PRMT5 expression levels of tissue array from normal cervix (n = 4), cervicitis (n = 3), carcinoma in situ (n = 3) and cervical cancer (n = 35) were analyzed using immunohistochemistry method. Values are presented as the mean ± SEM. (D) OS (left panel), PFI (middle panel) and DFI (right panel) of cervical cancer patients with different PRMT5 levels were analyzed from TCGA database. The "maxstat.test" function in R package maxstat was used to dichotomy gene expression, and all potential cutting points were repeatedly tested to find the maximum rank statistic, and then the patients were divided into the PRMT5-high group and the PRMT5-low group according to the maximum selected log-rank statistics, so as to reduce the calculated batch effect. Survival curves were generated using the Kaplan-Meier method, and the log-rank test was used to determine the significance of the differences. Univariate Cox regression model was used to calculate the hazard ratio (HR). *P < 0.05, **P < 0.01, and ***P < 0.001.