Figure 3.

PRMT5 deficiency in tumor cells affected tumor infiltrating T cells. Control cells and PRMT5 knockdown U14 cells were subcutaneously injected into 6-week-old female C57BL/6 mice (n = 5 for each group). Mice were euthanized at day 8 after inoculation. The tumor single cell suspension was prepared and analyzed by flow cytometry. (A) The percentage of CD4⁺ and CD8⁺ T cells in CD45⁺ cells, and the absolute number of the two T cell subsets in tumors. (B) Expression of IFN-γ, TNF-α and granzyme B in CD8⁺ T cells. (C) Expression of PD-1, TIM-3 and LAG-3 on the surface of CD8⁺ T cells. (D) Expression of IFN-γ, TNF-α and Foxp3 in CD4⁺ T cells. (E) Expression of PD-1, TIM-3 and LAG-3 on the surface of CD4⁺ T cells. Values are presented as the mean ± SEM. *P < 0.05 and **P < 0.01.