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. 2021 Aug 28;11(18):9162–9176. doi: 10.7150/thno.59605

Figure 4.

Figure 4

PRMT5 promoted STAT1 and PD-L1 expression. (A-B) Control cells and PRMT5 knockdown U14 cells were subcutaneously injected into 6-week-old female C57BL/6 mice. Mice were euthanized at day 8 after inoculation. (A) The tumor single cell suspension was prepared and the percentage of PD-L1 expression on CD45- cells was analyzed by flow cytometry (n = 5 for each group). (B) Immunohistochemical staining and statistical analysis of PD-L1 in tumor tissues (n = 3 for each group). (C-E) Control cells, PRMT5 knockdown U14 cells and PRMT5-overexpressing U14 cells were stimulated with IFN-γ for 24 h. PD-L1 expression on PRMT5 knockdown U14 cells (C) and PRMT5-overexpressing U14 cells (D) was analyzed using flow cytometry. (E) Real-time PCR experiments were used to test the expression of the indicated genes. (F) Western blot experiments were used to test the expression of the indicated proteins after IFN-γ stimulation for different time. Data are representative of at least two independent experiments (C-F). Values are presented as the mean ± SEM. *P < 0.05 and **P < 0.01.