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. 2021 Jul 31;11(18):8640–8659. doi: 10.7150/thno.61833

Figure 7.

Figure 7

Transient CSF1R blockade after lateral spinal cord hemisection in nonhuman primates does not affect microglial reactivity in the long term. Bright-field micrographs showing IBA1-positive microglia after SCI in untreated (A, E & G) and GW2580-treated (B, F & H) nonhuman primates rostral to the lesion site 3 months after SCI. Higher magnifications (E-H) of black insets in A & B. Line curves display quantification of IBA1-immunoreactivity in the dorsal funiculus on the ipsilateral (C) and the contralateral (D) sides of the injured spinal cord along the rostro-caudal axis. Quantifications of IBA1-immunoreactivity in segments rostral (I & K) and caudal (J & L) to the lesion. Quantification in the white matter (excluding the dorsal funiculus) (I & J) and the dorsal funiculus (K&L) at 3-months following SCI. IBA1-immunoreactivity was quantified on ipsilateral and contralateral sides of the spinal cord (I-L). Results for untreated nonhuman primates are in blue and GW2580-treated in green. Data are mean ± SEM per group. Student's unpaired t-test was used. Scale bars (A & B): 500 µm; (E & H): 100 µm. At least 40 sections (centered on the lesion site) per animal at 210µm intervals were analyzed. Number of Microcebus murinus: injured & untreated n = 5, injured & GW2580-treated for 2 weeks n = 5.