Figure 8.

Transient CSF1R blockade after lateral spinal cord hemisection in nonhuman primates does not affect muscle surface and neuromuscular junction density. Bright-field micrographs showing gastrocnemius-soleus-plantaris muscle complex of the hind limb located on the ipsilateral side of the spinal cord lesion in untreated (A) and GW2580-treated (C) Microcebus murinus. Black boxes in A & C correspond to higher magnification taken within the gastrocnemius muscle and presented in B & D, respectively. Graphs displaying quantitative assessments of the gastrocnemius muscle fiber surface area (E) and the density of neuromuscular junctions (F). In all graphs, results for untreated nonhuman primates are in blue and GW2580-treated are in green. Data are mean ± SEM per group. Student's unpaired t-test was used. Scale bars (A & C): 1 mm, (B & D): 100 µm. At least 20 sections per animal throughout the gastrocnemius muscle at 16 µm intervals were analyzed. Number of Microcebus murinus: injured & untreated n = 5, injured & GW2580-treated for 2 weeks n = 5.