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. 2021 Jul 31;11(18):8640–8659. doi: 10.7150/thno.61833

Figure 9.

Figure 9

Transient 1-week CSF1R blockade after SCI in mice induces transcriptional modification in microglia. RNA-seq analysis of FACS-isolated microglia from pooled (at least 2 animals and 16.000 cells) 1cm-spinal cord segments centered on the lesion site of SCI untreated and treated mice at 1 week after injury (end of the treatment) (A-B). We selected the same stringent cutoff as in our previous study [Fold Change (FC)≥2 and p-value with false discovery rate (FDR) ≤0.05] 7. Volcano plot (A). Heat map (B). In silico differential expression analysis (C-D). Comparison of the list of genes deregulated by the injury [identified in our previous study using the same parameters: male CX3CR1+/eGFP mice aged of 3 months, lateral hemisection of the spinal cord at T9 level, analysis of DE genes in microglia in a 1-cm segment centered on the lesion 1 week after injury (uninjured/SCI) 7) with the list of genes deregulated by GW2580 treatment in SC-injured mice (SCI-untreated / SCI-GW2580). Venn diagram (C). Fold changes of the 10 genes commonly deregulated in the comparison between (un-injured/SCI) and (SCI-untreated / SCI GW2580) (D).