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. 2021 Jul 25;11(18):8624–8639. doi: 10.7150/thno.48561

Figure 5.

Figure 5

Effects of Ang IV on the expressions of fibrosis-, apoptosis- and autophagy-associated markers and autophagy flux in cardiomyocytes. (A) Representative Western blot images of Col I, Col III, TGF-β1, Bax, Bcl-2 and Cl-caspase3 in 6 groups of cells. (B-F) Quantification of Col I, Col III, TGF-β1, Bax/Bcl-2 and Cl-caspase3 expressions in 6 groups of cells. (G) Representative Western blot images of LC3, Beclin1 and p62 in 6 groups of cells. (H-J) Quantification of LC3-II, Beclin1 and p62 expressions in 6 groups of cells. n=3 per group. (K) Representative images of immunofluorescent staining of LC3B in 3 groups of cells. (L) Quantification of immunofluorescent staining of LC3B in 3 groups of cells. n=5 per group. Ang IV: angiotensin IV; Cl-caspase3: cleaved caspase 3; Con: normal glucose control; Col I: collagen I; Col III: collagen III; HG: high glucose; HO: high osmotic control. *p < 0.05, **p < 0.01, ***p < 0.001.