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. 2021 Jul 25;11(18):8624–8639. doi: 10.7150/thno.48561

Figure 6.

Figure 6

Effects of Ang IV on FoxO1 phosphorylation and nuclear translocation in cardiomyocytes. (A) Representative Western blot images of pFoxO1 and FoxO1 expression in 3 groups of cells. (B) Quantification of pFoxO1/FoxO1 ratio in 3 groups of cells. n=3 per group. (C) Representative Western blot images of nuclear FoxO1 and nuclear internal control expressions in 3 groups of cells. (D) Quantification of nuclear FoxO1 in 3 groups of cells. n=3 per group. (E) Representative images of immunofluorescent staining of FoxO1 in 3 groups of cells. (F) Quantification of immunofluorescent staining of FoxO1 in 3 groups of cells. n=5 per group. Ang IV: angiotensin IV; Con: normal glucose control; HG: high glucose. *p < 0.05, **p < 0.01, ***p < 0.001.